Unlocking the Potential of CAR-T Therapy with Single-Cell Multi-Omics

In our latest study published on Cell Reports Medicine, led by Guerrero, Rill, et al., we dive deep into the cellular dynamics of CAR-T cell therapy, uncovering new insights using advanced single-cell multi-omics approaches. By analyzing both CARpos and CARneg T cells from patients with B-cell acute lymphoblastic leukemia (B-ALL), we discovered that the CD4/CD8 CARpos T-cell ratio plays a crucial role in predicting therapy outcomes and CAR-T cell persistence. We also identified pre-existing T-cell exhaustion as a key factor that may limit the efficacy of CAR-T treatments.

Interestingly, the expansion of γδ T cells at the peak of CAR-T cell expansion was strongly linked to better clinical outcomes. Our findings shed light on the complexity and diversity of the T-cell response in CAR-T therapy, offering promising biomarkers to predict patient responses and improve treatment strategies.

Read more about our findings here and how they could shape the future of immunotherapy!